The 3DO Opera platform does NOT have system wide region locks. There is no 'region' for which the consoles or software are tagged like many other systems. However, that does not mean all software will work across all consoles. There are compatibility issues which cause some software to fail to run. Whether these incompatibilities are accidental or purposeful and for what purpose they may have been introduced will depend on the title.

There are a few compatibility issues:

Game List

This list is not complete. New entries are added as discovered. Anything with a “Region” == “all?” have been shown to have the issue on at least one release but not yet confirmed for all releases.

Title Region Incompatibility
Brain Dead 13 all? 240p mode: Runs game logic twice as fast as it should.
Bust a Move U FZ-10 w/ Anvil chip: Similar to Road Rash
Dragon's Lair all? 240p mode: Runs game logic twice as fast as it should.
Escape from Monster Manor U 240p mode: Runs game logic twice as fast as it should.
Lemmings all? 240p mode: Runs game logic twice as fast as it should.
Novastorm all? 240p mode: Runs game logic twice as fast as it should.
Out Of This World all? 240p mode: Runs game logic twice as fast as it should.
Road Rash U FZ-10 w/ Anvil chip: The OS on the system is newer than the one on the disc so is loaded instead. The newer OS allocates a bit more ram which causes the kernel to be loaded at a different location which effectively reduces the amount of RAM available to the game. The game then loads and consumes a large portion of the RAM and when it launches the music player after the intro there isn't enough RAM for the music player and the interface indicates an error trying to play the music.
Space Ace all? 240p mode: Runs game logic twice as fast as it should.
Super Street Fighter 2 Turbo all? Not really a compatibility issue per se but the intro sequence often has audio desync'ed from the animation. This appears to be a bug in the intro sequence code as it does not happen in the Super Street Fighter II X - Grand Master Challenge (J,EU) demo (IE it is perfectly synced.)
Ultraman Powered J 240p mode: Fails to boot (black screen).
The Way of the Warrior E US NTSC console: Presents a screen which says “SORRY, THIS VERSION OF WAY OF THE WARRIOR IS FOR A EUROPEAN PAL MACHINE BUT YOUR MACHINE IS U.S. NTSC, SEE YOUR DEALER FOR THE CORRECT VERSION.”
Wolfenstein 3D E Any NTSC console: Presents a screen which says “WOLFENSTEIN 3DO CAN NOT RUN ON A NTSC 3DO.”
240p mode: Runs game logic twice as fast as it should.
Wolfenstein 3D U 240p mode: Runs game logic twice as fast as it should.
Yuu Yuu Hakusho J 240p mode: Crashes after starting story mode, or at character select screen.